Restriction Enzymes
Agilent Technologies DPN I RESTRICTION ENZYME
Dpn I Restriction Enzyme, is negative for nonspecific nucleases. It is used when cloning into multiple cloning site of pBluescript II phagemid vector. It includes an optimal (10X) buffer and/or a 10X concentrate of Universal buffer.
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
New England Biolabs, Inc. SalI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^TCGAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. AleI-v2 – 2500 units
AleI-v2 is an engineered and improved version of AleI that recognizes the sequence CACNN^NNGTG.
New England Biolabs, Inc. BspQI HF-2500 units
A Type IIS restriction endonuclease that recognizes the sequence GCTCTTC(1/4). An E. coli strain that carries the modified cloned BspQI gene from Bacillus sphaericus. BspQI-HF is an engineered and improved version of BspQI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
Vector Biolabs SRF-Luc Adenovirus (Ad-SRF-Luc)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
ABclonal Technology BspQI
5...G C T C T T C (N)1...3 3...C G A G A A G (N)4...5 Isoschizomers: LguI, SapI, PciSI
ABclonal Technology SacII
5...C C G C/ G G...3 3...G G/ C G C C...5 Isoschizomers: Cfr42I KspI Sfr303I SgrBI
Cayman Chemical Myrca rubra bark Myrctrn 50mg
A flavonoid with diverse biological activities; active against B. subtilis, E. coli, K. pneumoniae, S. aureus, and C. albicans (MIC = 0.25 mg/ml for all); inhibits AChE and α-glucosidase in vitro (IC50s = 65 and 79 μg/ml, respectively); inhibits LPS-induced production of TNF-α and enhances LPS-induced RANTES production in RAW 264.7 cells at 0.2 and 1 mM
Cayman Chemical NItrate Reductase Enzyme Prep
A VEGFR1 VEGFR2 and EGFR inhibitor (IC50s 0.7 0.03 and 0.4 UM respectively) selective for these kinases over PDGFRB FGFR1 and HER2 (IC50s 3.4 >100 and >100 UM respectively) and MEK Cdk2 IGF-1R Akt and Tie2 (IC50s >10 >10 >20 >20 and >100 UM respectively) selectively inhibits VEGF- or EGF-induced proliferation of HUVECs over FGF-induced proliferation of HUVECs (IC50s 0.05 0.05 and 1.5 UM respectively) reduces tumor volume in a Calu-6 NSCLC mouse xenograft model at 100 mg/kg inhibits training-induced increases in collateral blood flow and white gastrocnemius muscle capillarity in exercise-trained rats